Brucella antibodies - serum

Last updated: Thursday, 08, April, 2004

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Item Process
Specimen

5-10 mL blood in plain tube. Acute and convalescent (3-4 weeks) samples.

Method

Bacterial agglutination, Coombs anti-human globulin test, CFT, EIA.

Application

PUO with history of relevant animal exposure.

Interpretation

A bacterial agglutination titre of >160 suggests past or recent infection; a four-fold rise in titre between acute and convalescent samples is evidence of recent infection.

Blocking antibodies can lead to false negative results at low dilutions of serum.

Reference

Chu MC and Weyant RS. In: Murray PR et al eds. Manual of Clinical Microbiology. 8th ed. ASM Press 2003.