Leptospira sp antibodies - serum

Last updated: Wednesday, 24, March, 2004

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Item Process
Specimen

5-10 mL blood in plain tube, acute and convalescent specimens required.

Method

Microagglutination, EIA.

Application

Investigation of suspected leptospirosis (eg, fever, hepatitis, renal failure).

Interpretation

IgM is detectable by EIA within the first week of the disease. Since it may persist for many months IgG seroconversion is required to confirm a diagnosis of recent infection.

A four-fold rise in microagglutination titre, or a titre of ≥800, in a suggestive clinical setting is strong evidence of infection.

Microagglutination serology can be used to identify the serovar likely to be causing infection.

Reference

Winslow WE et al. J Clin Microbiol 1997; 35: 1938-1942.