Corneal scrapings/biopsy

Last updated: Saturday, 03, April, 2004

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Item Process

Scraping or biopsy from inflamed or ulcerated cornea, processed for microscopy and culture at bedside.


Gram stain, DFA and/or PCR testing for chlamydia, culture for bacteria, chlamydia, fungi, viruses and amoebae. Histology and EM.

See also Chlamydia trachomatis detection.


Diagnosis of cause of keratitis or corneal ulcer, especially if long standing.


The cause of keratitis or corneal ulcer can be established by demonstration of the organism involved (eg, Pseudomonas sp, herpes simplex, fungi, or acanthamoebae) in corneal tissue.


Wilhelmus K et al. In: Specter SC ed. Cumitech 13A  ASM Press 1994.