Collagen binding assay
Last updated: Wednesday, 31, March, 2004
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| Item | Process |
|---|---|
| Specimen | 4.5 mL blood added to 0.5 mL citrate. |
| Method | Immunoassay (EIA; ELISA) with measure of binding of VWFAg to collagen-coated microtitre plates. Abbreviated VWF:CB. |
| Reference Interval | Generally 50-200% of the level in pooled normal plasma. |
| Application | Additional functional VWF complementary to the ristocetin cofactor assay in the investigation of a possible bleeding disorder: diagnosis of von Willebrand’s disease and its variant forms. See von Willebrand factor (Ristocetin cofactor; VWF:RCo) and von Willebrand factor multimers. |
| Interpretation | Levels are reduced in classical (type I) von Willebrand’s disease, usually to a similiar extent as the reduction in factor VIII and VWF:Ag. Levels of VWF (collagen binding and ristocetin cofactor) are reduced disproportionately to VWF:Ag in type 2 (A and B) VWD. In Type 2M VWD, levels of VWF:CB and/or VWF:RCo (that is, either or both) may be reduced disproportionately to VWF:Ag, depending on the specific VWF defect represented. See also von Willebrand's disease; von Willebrand factor antigen (VWF:Ag) and Ristocetin cofactor. |
| Reference | Favaloro EJ. Clin Haematol 2001; 14: 299-319. Favaloro EJ. Semin Thromb Hemost 2002; 28: 191-202. Favaloro EJ. Haemophilia 2002; 8: 828-831. |