Direct antiglobulin test (DAT)
Last updated: Thursday, 01, April, 2004
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| Item | Process |
|---|---|
| Specimen | 5 mL blood in EDTA tube. |
| Method | Polyspecific anti-human globulin serum added to patient’s washed red cells. The test is positive if agglutination is observed. |
| Application | Investigation of possible immune haemolysis. Detection of antibody and/or complement on red cell surface. |
| Reference | Ford D. Aust J Med Lab Sci 1995; 16: 67-70. |