Haemoglobin - urine

Last updated: Thursday, 01, April, 2004

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Item Process

Freshly voided urine collected without preservative.


Spectroscopic examination or urinalysis to detect haem pigment.

Urine microscopy to exclude haematuria.

EPG or IFE to distinguish haemoglobin from myoglobin.

Reference Interval

Not detected in normal urine.


History of red or dark urine; findings on FBC and blood film suggesting intravascular haemolysis; investigation of immediate haemolytic reaction during blood transfusion.

See also Myoglobin - urine.


Haemoglobinuria indicates intravascular haemolysis;
release of haemoglobin in amounts exceeding the binding capacity of haptoglobins; and
consequent glomerular filtration of haemoglobin and presence in urine.


Dacie JV and Lewis SM. Practical Haematology. 8th ed. Churchill Livingstone 1995.