Pyruvate kinase - red cell

Last updated: Friday, 02, April, 2004

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Item Process
Specimen

5 mL blood in ACD, lithium heparin, or EDTA tube.

Method

Washed red cells are lysed. Assay performed at 37°C and results expressed as enzyme activity per gram of haemoglobin.

Reference Interval

Check with laboratory.

Application

Investigation of non-spherocytic haemolytic anaemia.

Specific enzyme assay confirms deficiency.

Interpretation

Low levels confirm red cell pyruvate kinase deficiency.

Reference

Valentine WN et al. Ann Intern Med 1985; 103: 245-248.