Bilirubin - amniotic fluid

Last updated: Thursday, 08, April, 2004

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Item Process
Specimen

2 mL amniotic fluid.  Protect from light.

Method

Spectrophotometry.

Reference Interval

Varies with gestational age.

Some laboratories express the result as µmol/L, others as an ODD450 (optical density difference at 450 nm).

Application

Investigation of pregnancies at risk for Haemolytic disease of the newborn.

Interpretation

Increased levels indicate haemolysis.

A number of different graphs have been published correlating degree of risk with amniotic fluid bilirubin level at various gestational ages.

Bleeding into the amniotic fluid invalidates results.

Reference

Oepkes D. Eur J Obstet Gynecol Reprod Biol 2000; 92(1): 83-89.