Haemoglobin F (HbF) - flow cytometry
Last updated: Wednesday, 28, April, 2004
5 mL blood in EDTA tube.
Cytoplasmic staining for HbF with anti-HbF monoclonal antibody.
Fetal erythrocytes stain positively. Normal adult erythrocytes do not.
Detection and quantitation of feto-maternal haemorrhage.
Studies in th thalassaemias.
Normal adult cells are negative, adult fetal cells are weak positive (HbF~10% in each cell) and true fetal cells are bright positive (100% HbF).
The proportion of fetal cells is calculated as a percentage of all red cells. The flow cytometric method provides a more accurate and objective quantitation of fetal cells in the maternal circulation.
Olsen SH et al. Lab Haematol 1996; 1:7.