Breast Biopsy - Oestrogen receptors (ER) and Progesterone receptors (PR)
Last updated: Wednesday, 28, November, 2007
Formalin-fixed paraffin embedded breast tissue. For optimum results tissues should be sliced to facilitate fixation.
Immunohistochemical staining of processed tissue with positive and negative controls.
To detirmine if tumours are potentially responsive to anti-oestrogen treatment.
% of positive nuclei Score
No staining 0
Weak staining 1
Moderate staining 2
Strong staining 3
The distribution and intensity of scored are added to give a score out of 8.
Leake R, Barnes D, Pinder S, et al. Immunohistochemical detection of sterioud receptors in breast cancer: a workin protocol. J Clin Pathol 53: 634-635, 2000